5f0ded59b9d32b06f17b9040
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
True
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9041
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
78 % ee S
True
Medium
12
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9042
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
95 % ee S
True
Medium
22
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9043
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
84 % ee S
True
Low
8
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9044
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
87 % ee S
True
Low
1
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9045
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
99 % ee S (2S)
True
Medium
10
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9046
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
98 % ee S (2S, 5S)
True
Medium
21
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9047
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
92 % ee S
True
Low
4
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9048
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
99 % ee S (2S, 3S)
True
Medium
47
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9049
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
64 % ee S (2R, 3S)
True
Medium
26
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b904a
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
80 % ee S (3S, 4S)
True
Low
2
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b904b
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
90 % ee S
True
Medium
12
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b904c
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
90 % ee
True
Medium
53
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b904d
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
96 % ee S
True
Medium
18
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b904e
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
99 % ee S
True
Low
9
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b904f
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
99 % ee S
True
Medium
28
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9050
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
99 % ee S
True
Low
9
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9051
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
True
Low
1
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9052
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
87 % ee S
True
Low
1
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24
5f0ded59b9d32b06f17b9053
Alkene oxidation
SMO
RhSMO
https://doi.org/10.1002/adsc.201400383
5f0ded59b9d32b06f17b903e
91 % ee S
True
Medium
17
25
7.5
water, hexyl acetate
20 % w/v whole cells in 50 mM potassium phosphate buffer containing 1 mM NAD+ and 0.2 mM FAD (1 mL). Cell suspension, 200 mM organic solvent solution of the substrate (1 mL) and 0.12 mL 2-propanol transferred to 50 mL tube. Tube was then filled with pure oxygen, reaction performed at 300 rpm
Whole cells. LSADH was used (co-expressed) to recycle NADH cofactor
2.12
whole cell
200
100
24