Activity data - for NHase enzymes

Click on a row for more information and links to related data

Reaction
Enzyme type
Enzyme name
Data source
Substrate 1 SMILES
Product 1 SMILES
Selectivity
Binary
Categorical
Conversion (%)
Specific activity (U/mg)
Temperature
pH
Solvent
Other conditions
Reaction volume (ml)
Biocatalyst Formulation
Biocatalyst Concentration (mg/ml)
Substrate 1 conc (mM)
Conversion time (hrs)
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Medium
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Medium
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Medium
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
False
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Low
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Medium
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Low
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
False
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Medium
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Medium
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Low
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
False
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
Low
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
False
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
False
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
False
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
High
100
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
High
100
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
24
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
High
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
120
Nitrile hydrolysis (CONH)
NHase
RrNHase
Mashweu et al, 2020, Molecules
 
True
High
30
7.6
water
1:1 mass ratio purified NHase:substrate, 10 mg NHase, 10 mg substrate dissolved in 200 uL acetone or methanol, 50 mM Tris buffer, 2 mL reaction volume, 199 rpm
2
purified
5
 
120