Activity data - for ADH(FAD) enzymes

Click on a row for more information and links to related data

Reaction
Enzyme type
Enzyme name
Data source
Substrate 1 SMILES
Product 1 SMILES
Binary
Temperature
pH
Solvent
Other conditions
Notes
Reaction volume (ml)
Biocatalyst Formulation
Biocatalyst Concentration (mg/ml)
Substrate 1 conc (mM)
Conversion time (hrs)
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12
Primary alcohol oxidation
ADH(FAD)
PpAlkJ
Wu et al, 2016, Nat. Commun.
True
30
8
water, hexadecane
10 g cdw/l E. coli whole cells containing all cascade enzymes, 200 mM potassium phosphate buffer (0.5 % glucose), n-hexadecane (1:1)
Biphasic system, substrate concentration quoted for concentration in organic phase
2
whole cell
10
20
12